Serotiny (a trait that allows plants to store seeds in closed cones until an environmental stimulus triggers dispersal) is a common trait for pine species growing in pyrogenic ecosystems. However, serotiny is not a fixed trait, and partial serotiny has been observed within Ocala sand pine (Pinus clausa var. clausa) stands in Florida. We studied serotiny in 97 Ocala sand pine individuals, representing a range of recent fire history, tree age, and canopy seed bank size at Archbold Biological Station (ABS) in south-central Florida. We counted the number of serotinous and non-serotinous cones in each canopy, quantified recent fire history, measured tree diameters, and estimated tree ages. Contrary to our expectations, serotiny did not vary with fire frequency, time-since-fire, or fire severity. However, non-fire induced opening of cones occurred more often in older, more cone-laden individuals, which showed that a bet-hedging strategy might occur in Ocala sand pine. These patterns could also reflect that the physiological trade-offs associated with maintaining a large canopy seed bank are driving non-fire induced opening of cones at small spatial scales. Accordingly, long-term monitoring is needed to unravel whether partial serotiny in Ocala sand pine is driven by a bet-hedging strategy or physiological constraints.