This experiment tests the effects of early canopy development by asexually regenerating aspen (Populus tremuloides Michx.) on conifer recruitment after fire in central Alaska. The establishment and growth of three conifer species were observed in response to aboveground removal of aspen suckers for three seasons after burning by wildfire. Of the three species, Pinus contorta Dougl. ex Loud. had the most widespread seed germination and showed the strongest negative response to the presence of the aspen canopy. Picea mariana (Mill.) BSP and Picea glauca (Moench) Voss had low germination and weak or neutral responses to aspen removal. Seedlings of all species accumulated more biomass in the removal treatment. Results from the experiment suggest that competition by aspen early after disturbance can significantly reduce conifer recruitment and growth, an effect that may reinforce the long-term dominance of aspen in asexually regenerating stands.