Canopy gaps play an important role in forest vegetation dynamics when fire return intervals are long. However, there is little known about the role of gaps in the development of forest stands that initially dominate following stand-replacing disturbance. We investigated gap disturbance during the breakup of trembling aspen (Populus tremuloides) stands at two scales: at the stand level we quantified gap fraction and gap size as stand development proceeds; at the gap level we determined causes of gap-maker mortality and evaluated resulting gap-maker structure and decay as stand development proceeds. We also evaluated the impact that gaps have on stand transition by quantifying the abundance and growth of juvenile trees in gaps of different sizes and ages. Ten stands between 60 and 120 years since fire in northeastern Ontario, Canada, were sampled using line intersect transects. Gap fraction doubled (~18-36%) and mean gap size was more than four times greater (~45-200 m2) over the time period. Standing dead gap makers in early states of decay were most frequent in young stands, whereas snapped gap makers in various states of decay were most frequent in old stands. Infection by fungal pathogens was the most frequent cause of mortality (56%) and was not related to time since fire. Balsam fir (Abies balsamea) was the most abundant juvenile recruit. However, transition probabilities for tree species were independent of gap type. These results indicate that gap creation is frequent during early stages of stand development in boreal forests; however, it is unlikely that successional trajectory is affected by their formation.