Resource Catalog
Document
Isolated zygotic embryos and female gametophytes containing zygotic embryos were cultured on MSG and DCR basal media, supplemented with three different carbon sources added individually to the medium at four levels each. The media also contained various levels of 2,4-dichlorophenoxy acetic acid (2,4-D) and N6-benzyladenine (BA). Embryogenic tissue extruded from female gametophytes during 4 weeks in culture on media containing either glucose or maltose or sucrose. Embryogenic tissue initiation was most frequently from explants collected on July 14, 1992, when the zygotic embryos within the female gametophytes were precotyledonary. A total of 33 embryogenic cultures were initiated from 944 explants cultured. One of 192 explants cultured on basal media with no growth regulators produced embryogenic tissue. The embryogenic tissue showed numerous somatic embryos at stages 1 and 2 of development, corresponding to their zygotic embryo counterparts. © National Research Council of Canada, NRC Research Press. Abstract reproduced by permission.
Cataloging Information
- Canada
- carbon
- cones
- coniferous forests
- forest management
- Larix decidua
- longleaf pine
- Mississippi
- mortality
- pH
- Picea abies
- pine forests
- Pinus palustris
- Pinus ponderosa
- Pinus radiata
- Pinus taeda
- plant diseases
- plant growth
- plant physiology
- Pseudotsuga menziesii
- seeds
This bibliographic record was either created or modified by Tall Timbers and is provided without charge to promote research and education in Fire Ecology. The E.V. Komarek Fire Ecology Database is the intellectual property of Tall Timbers.