Document


Title

Changes in cultured dermal fibroblasts during early passages across five wild bird species
Document Type: Journal Article
Author(s): E. A. Calhoon; M. K. Miller; A. G. Jimenez; J. M. Harper; J. B. Williams
Publication Year: 2013

Cataloging Information

Keyword(s):
  • American robin
  • American robin
  • Anas clypeata
  • Anas clypeata
  • Anas platyrhynchos
  • Anas platyrhynchus
  • bobwhite quail
  • bobwhite quail
  • Colinus virginianus
  • Colinus virginianus
  • fibroblast
  • fire dependent species
  • game birds
  • lipid
  • mallard
  • mallard
  • mourning dove
  • mourning dove
  • nongame birds
  • northern shoveler
  • northern shoveler
  • passage
  • phospholipid
  • physiology
  • plasmalogen
  • threatened and endangered species (animals)
  • Turdus migratorius
  • Turdus migratorius
  • wildlife management
  • Zenaida macroura
  • Zenaida macroura
Topic(s):
Record Maintained By:
Record Last Modified: June 1, 2018
FRAMES Record Number: 51506
Tall Timbers Record Number: 28392
TTRS Location Status: Not in file
TTRS Call Number: Available
TTRS Abstract Status: Okay, Fair use, Reproduced by permission

This bibliographic record was either created or modified by the Tall Timbers Research Station and Land Conservancy and is provided without charge to promote research and education in Fire Ecology. The E.V. Komarek Fire Ecology Database is the intellectual property of the Tall Timbers Research Station and Land Conservancy.

Description

With the advent of the usage of primary fibroblasts in comparative and evolutionary biology, it is important for researchers to know the extent to which cells might be altered during the culturing process and how much species might differ in response to cell culture. We compared early changes in cell size and lipid composition of primary dermal fibroblasts grown at physiologically relevant oxygen concentrations (5% O2) from wild-caught species of birds. Fibroblasts from American Robins (Turdus migratorius L., 1766) and Bobwhite Quails (Colinus virginianus (L., 1758)) increased in size early in the culture process and cells from all five species of wild-caught birds exhibited changes in lipid-class composition. The two most common phospholipids, phosphatidylcholine and phosphatidylethanolamine, increased in concentration in all species between early passages and later passages of fibroblasts. Some less abundant lipid species, such as cardiolipin and sphingomyelin, exhibited similar concentrations in all three passages that we measured. Other lipid classes, such as cholesterol, increased in some species in later passages and decreased in others. Although results may vary with cell-culture conditions, this study points to a need for researchers comparing multiple species to take precautions when using cell culture, such as experimenting on the earliest possible passage of cells.

Citation:
Calhoon, E. A., M. K. Miller, A. G. Jimenez, J. M. Harper, and J. B. Williams. 2013. Changes in cultured dermal fibroblasts during early passages across five wild bird species. Canadian Journal of Zoology, v. 91, no. 9, p. 653-659.