The demand for container longleaf pine (Pinus palustris Mill.) planting stock is increasing across the Lower Gulf Coastal Plain. Poor-quality seeds and seedling losses during nursery culture further constrain a limited seed supply. Improved seed efficiency will be necessary to meet the need for increased seedling production. We evaluated seed presowing treatments and seedling fungicidal applications in container longleaf pine seedling operations to determine if efficiency of seedling production could be improved. Application of treatments to reduce pathogenic fungi on seed and in seedling culture significantly increased plantable container stock.