Document


Title

Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa)
Document Type: Journal Article
Author(s): Jill A. Hoff; Ned B. Klopfenstein; Geral I. McDonald; Jonalea R. Tonn; Mee-Sook Kim; Paul J. Zambino; Paul F. Hessburg; Jack D. Rogers; Tobin L. Peever; Lori M. Carris
Publication Year: 2004

Cataloging Information

Keyword(s):
  • Byssochlamys nivea
  • Douglas-fir
  • endophytes
  • FFS - Fire and Fire Surrogate Study
  • fungi
  • Pinus ponderosa
  • ponderosa pine
  • Pseudotsuga menziesii
  • roots
Region(s):
JFSP Project Number(s):
99-S-01
Record Maintained By:
Record Last Modified: April 7, 2016
FRAMES Record Number: 233

Description

The fungal community inhabiting large woody roots of healthy conifers has not been well documented. To provide more information about such communities, a survey was conducted using increment cores from the woody roots of symptomless Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa) growing in dry forests on the eastern slope of the Cascade Mountains in Washington state, USA. Fungal isolates were cultured on standard media, and then were identified using a combination of molecular and morphological methods. Fungal genera and species identified in this study will provide baseline data for future surveys of fungal endophytes. Examination of internal transcribed spacer (ITS1 and ITS2) and 5.8S rDNA sequences and morphology of cultured fungi identified 27 fungal genera. Two groups predominated: Byssochlamys nivea Westling (20.4% of isolations) and Umbelopsis species (10.4% of isolations). This is the first report of B. nivea within large woody roots of conifers. Both taxa have been previously identified as potential biological control agents. Although some trends were noted, this study found no significant evidence of host species or plant association effects on total recovery of fungal endophytes or recovery of specific fungal taxa.

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Citation:
Hoff, J.A.; Klopfenstein, N.B.; McDonald, G.I.; Tonn, J.R.; Kim, M.-S.; Zambino, P.J.; Hessburg, P.F.; Rogers, J.D.; Peever, T.L.; and Carris, L.M. 2004. Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa). Forest Pathology 34(4):255-271.